University of Minnesota
BioTechnology Institute
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BRC Services


At the Biotechnology Resource Center we have experience with fermentations using a wide variety of bacteria, yeast, and fungi. The services we offer include:
- Cultivation of bacteria, yeast, fungi, and algae
- Strain development an optimization
- Process development and optimization
- Pilot-scale production
- Process trouble-shooting
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Downstream processing

The Biotechnology Resource center offers capabilities for the recovery and purification of cells, proteins, as well as primary and secondary metabolites from the bench-scale to the pilot-scale. Services include:
- Recovery and purification of proteins and small molecules
- Cell disruption
- Inclusion body preparations
- Product harvest and concentration by micro- and ultrafiltration
- Protein refolding
- Freeze drying
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Protein expression

Our work in protein expression integrates most of the services described above. Our primary expression hosts are E. coli, Pichia pastoris, and Saccharomyces cerevisiae. A complete protein expression project would comprise the following steps:
- Cloning target gene into chosen expression vector
- Creation of fusion proteins, if desired
- Initial protein expression analysis on a small scale
- Expression optimization specific to host organism
- Scale-up of fermentation or cell culture to desired process size
- Development and optimization of protein recovery and purification strategy
- Scale-up of recovery and purification process
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GMP capabilities

- Fully established quality system
- Production Phase I and Phase IIA clinical trial material
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BRC services

For researchers at the University and in industry, the Biotechnology Resource Center offers customized research & development services as well as limited production services in the following areas:

If you need advice on refining your experiments or want us to fully execute your project, our team of experts will be glad to assist you. Even if your project does not clearly fall in any of the areas described below, do not hesitate to contact us, since we are constantly striving to expand our portfolio of services.