Recent Publications (Last seven years)

Griffith, C.,J. Morris, M. Robichaud, M.J. Annen, A.V. McCormick, and M.C. Flickinger. "Fluidization Characteristics of and Protein Adsorption by Porous Zirconium Oxide Particles in a Fluidized-Bed". J. Chrom. 776: 179-195 (1997).

Reeder, D.H., J. Li, P.W. Carr, M.C. Flickinger and A.V. McCormick. Models for Polybutadiene Pore Wall Coatings in Porous Zirconia. J. Chrom.760: 71-79 (1997).

Robichaud, M., A.N. Sathyaral, P.W. Carr, A.V. McCormick and M.C. Flickinger. "An Improved Oil Emulsion Synthesis Method for Large, Porous Zirconia Particles for Packed or Fluidized-Bed Protein Chromatography". Sep. Sci. Technol. 32: 2547-2559 (1997).

Lin, G., P.R. Pentel, W.L. Shelver, D.E. Keyler, C.A. Ross, Y. Heida, C.A. Pennell, M.C. Flickinger and M.P. Murtaugh. "Bacterial Expression and Characterization of an Anti-Desipramine Single Chain Antibody Fragment". Int. J. Pharmacol. 18: 729-738 (1997).

Cue, D., H. Lam, R.S. Hanson and M.C. Flickinger. "Genetic Manipulation of Bacillus methanolicus, a Gram Positive Thermotolerant Methylotroph". Appl. Environ. Microbiol. 63:1406-1420 (1997).

Mullick, A. and M.C. Flickinger. Comparison of Fluoride Modified Zirconia with Ceramic Hydroxyapatite for Preparative Scale Purification of Immunoglobulins from Serum Albumin. Prep. Biochemistry. 32: 2547-2559 (1998).

Mullick, A., C.M. Griffith and M.C. Flickinger. Expanded and Packed Bed Albumin Adsorption on Fluoride Modified Zirconia. Biotechnol. Bioeng. 60: 333-340 (1998).

Flickinger, M.C., A. Mullick and D.F. Ollis. A Laboratory Scale Non-Woven Biocatalytic Film Biofilter. Biotechnol. Prog. 14: 664-666 (1998).

McNeff, C., E. Almlöf, M.C. Flickinger and P.W. Carr "Removal of Pyrogenic Lipopolysaccharide from Insulin using PEI-Coated Porous Zirconia Particles". Anal. Biochem. 274: 181-187 (1999).

Martinez, M.B., M.C. Flickinger and G.L. Nelsestuen. Steady State Enzyme Kinetics in the Escherichia coli Periplasm: A Model of a Whole-Cell Biocatalyst. J. Biotechnol. 71: 59- 66 (1999).

Flickinger, M.C., A. Mullick and D.F. Ollis. Use of Azocasein Release to Characterize the Sealant Coat Porosity of Fibers Coated with Biocatalytic Films. Biotechnol. Prog. 15:383-390 (1999).

Lyngberg, O.K., V. Thiagarajan, D. Stemke, J. Schottel and M.C. Flickinger. A Patch Coating Method for Preparing Biocatalytic Films of Escherichia coli. Biotechnol. Bioeng. 62: 44-55 (1999).

Lyngberg, O.K., D. J. Stemke, J.L. Schottel and M.C. Flickinger. A Simple Single use Luciferase Based Mercury Biosensor using Latex-Film Immobilized Escherichia coli HB101. J.Ind. Microbiol. Biotechnol. 23: 668-676 (1999).

Thiagarajan, V.S., Z. Huang, L.E. Scriven, J.L. Schottel and M.C. Flickinger. Microstructure of an E. coli Biocatalytic Latex Coating Elucidated by Cryogenic Scanning Electron Microscopy. J. Coll. Interfac. Sci. 215: 244-257 (1999).

Huang, Z., V.S. Thiagarajan, O.K. Lyngberg, L.E. Scriven and M.C. Flickinger. Microstructure Evolution in Polymer Latex For Whole-Cell Biocatalyst Application. J. Coll. Interfac. Sci.215: 226-243 (1999).

Mullick, A. and M.C. Flickinger. Expanded Bed Adsorption of Human Serum Albumin from Very Dense Saccharomyces cerevisae Suspensions on Fluoride Modified Zirconia. Biotechnol. Bioeng. 65: 282-290 (1999).

Reeder, D.H., P.W. Carr, M.C. Flickinger, and A.V. McCormick. Diffusion of Non-Adsorbing Polymers within Hierarchically Structured Colloidal Aggregates. J. Colloid. Interfac. Sci. 226: 277-285 (2000).

Lyngberg, O.K., C.P. Ng, V. Thiagarajan, L.E. Scriven and M.C. Flickinger. Engineering the Microstructure and Permeability of Thin Multilayer Latex Biocatalytic Coatings Containing E. coil. Biotechnol. Prog. 17: 1169-1179 (2001).

Martinez, M.B., M.C. Flickinger, L.A. Higgins, T. Krick and G.L. Nelsestuen. Reduced Outer Membrane Permeability of Escherichia coli O157:H7: Role of Modified Outer Membrane Porins and Theoretical Function in Resistance to Antimicrobial Agents. Biochemistry 40:11965-11974 (2001).

Pluschkell, S.B. and M.C. Flickinger. Dissimilation of 13 C-Methanol by Continuous Cultures of Bacillus methanolicus MGA3 at 50°C Studied by 13 C NMR and Isotope Ratio Mass Spectrometry. Microbiology 148:3223-3233 (2002).

Brautaset, R., M.D. Williams, R. Dillingham, C. Kaufmann, E. Crabbe and M.C. Flickinger. “The Bacillus methanolicus Citrate Synthase II Gene, cit Y: Analysis of its Role in Regulating the Secretion of Glutamate in L-Lysine Overproducing Mutants”. Appl. Environ . Microbiol. 69: 3986-3995 (2003).

Brautaset, T., Ø.M. Jacobsen, M.C. Flickinger , S. Valla, and T. E. Ellingsen. “Plasmid Dependent Methylotrophy in Thermotolerant Bacillus methanolicus ” J. Bacteriol. 186: 1229-1238 (2004).

Komives, C.F., L.Y. Cheung, S.B. Pluschkell and M.C. Flickinger. “Growth of Bacillus methanolicus in Sea-Water-Based Media” J. Ind. Microbiol. Biotechnol. (2005) (in press).

Lyngberg, O.K., C. Solheid, S. Charaniya, Y. Ma, V. Thiagaragan, L.E. Scriven and M.C. Flickinger “Permeability and Reactivity of Thermotoga maritima in Latex Polymer Blend Coatings at 80ºC: A Model High Temperature Biocatalytic Coating” Extremophile (2005) (in press).

Mota, M., A. Yelshin, and M.C. Flickinger. “Modeling Diffusivity in Polymeric Multi-layer Membranes Containing Microbial Cells” (submitted).

Recent Patents Issued (back to the top)

D.A. Mills and M.C. Flickinger.
Bacillus MGA3 Diaminopimelate Decarboxylase.
U.S. 5,426,052 June 20, 1995.

R.S. Hanson, M.C. Flickinger, F.J. Schendel and M. Guettler.
Production of Amino Acids Using Methylotrophic Bacillus.
European Patent 0422187. September 20, 1995.

R.S. Hanson, M.C. Flickinger, F.J. Schendel and M.V. Guettler.
Production of Amino Acids Using Methylotrophic Bacillus.
German National Patent 69022522.9-08. February, 1996.

M.C. Flickinger, C. Griffith, M. Robichaud, J. Morris, M. Annen, C. Dunlop, A. McCormick and P.W. Carr.
Protein Adsorption by Very Dense Porous Zirconium Oxide Particles in Expanded Beds.
U.S. 5,837,826. November 17, 1998.

R.S. Hanson, M.C. Flickinger, F.J. Schendel, M.V. Guettler.
Production of Amino Acids by Methylotrophic Bacillus.
Canadian Patent 2,030,529. October 19, 1999.

M.C. Flickinger, C. Griffith, M. Robichaud, J. Morris, M. Annen, C. Dunlop, A. McCormick and P.W. Carr.
Protein Adsorption by Very Dense Porous Zirconium Oxide Particles in Expanded Beds.
U.S. 6,036,861. March 24, 2000.

R.S. Hanson, M.C. Flickinger, P. Olson, W. Hur, N. Al-Tahoo, C. Bremmon.
Production of Amino Acids using Auxotrophic Mutants of Methylotrophic Bacillus
U.S. 6,110,713. August 29, 2000.

R.S. Hanson, M.C. Flickinger, F.J. Schendel, M.V. Guettler. Production of Amino Acids using Auxotrophic Mutants of Methylotrophic Bacillus.
U.S. 6,261,825. July 17, 2001.

D.S. Sherman, P.A. August and M.C. Flickinger. Genes Encoding Resistance to DNA Bioreductive Alkylation of Cleaving Agents and Method for Identification of Compounds that Inhibit Resistance to DNA Bioreductive Alkylating and Cleaving Agents U.S. 6,524,812, February 25, 2003 .